In
Vitro Research and Development
PRECOS
offers medium throughput multi-compound screening
in over 100 human, mouse and rat cell lines,
including unique human colorectal, gastric,
pancreatic and oesophageal cell lines.
PRECOS
in vitro research and development assays provide
highly reproducible results, rapid data production
and multiple drug screening and can be offered
as a stand-alone activity, or in support of
a wider programme.
Cell
Proliferation and Viability Assays
The following industry standard
assays are available:
- Tetrazolium
uptake: a colorimetric
method for determining the number of viable
cells in proliferation, cytotoxicity or
chemosensitivity assays.
- Lactase dehydrogenase
(LDH): a widely accepted standard
for the assessment of cell membrane integrity.
- Tritiated (3H)
thymidine: Incorporation into cellular
DNA is one of the most robust and sensitive
measures of cell number available.
Apoptosis
Assays
The ability to detect apoptosis
is critical in many experimental scenarios.
PRECOS can evaluate the onset of apoptosis
using two industry standard markers.
- Mitochondrial
permeability transition (MPT):
providing an early indication of cellular
apoptosis.
- Caspase 3 enzyme
induction: triggered in response
to pro-apoptotic signals.
Radioligand
Binding Assays
Saturation and competitive
binding analysis are useful tools in the analysis
of G protein-coupled receptors (GPCRs) and
their ligand interactions for receptor model
selection, ligand screening and target selection.
- Saturation analysis:
Using appropriate cold and hot ligands,
receptor affinity (KD) and density (BMAX)
can be determined in both cell membrane
and whole cell systems.
- Competition analysis:
Using an appropriate receptor source and
competing radioligand, the equilibrium dissociation
constant (KI) indicating ligand affinity
can be determined.
Diagnostic/Biomarker
Detection
Based on the Enzyme-linked
Immunoabsorbent assay (ELISA), PRECOS can
provide high sensitivity (nano-and pico-molar
level) analysis of serum, media and cell extracts
for a comprehensive range of biological targets/markers.
